Corneal Regenerative Medicine: Methods and Protocols by Tor Paaske Utheim (auth.), Bernice Wright, Che J Connon

By Tor Paaske Utheim (auth.), Bernice Wright, Che J Connon (eds.)

Continuous regeneration of the cornea is critical to take care of this tissue within the obvious country that's crucial for imaginative and prescient. treatment for fix of the broken anterior cornea is at the moment addressed during the transplantation of donor corneas or the supply of limbal epithelial stem cells (LESC) to the ocular floor utilizing amniotic membrane (AM) as a assisting scaffold. study at the bioengineering of corneal equivalents as substitute tissue is underway to strengthen doable corneal prosthetics. Corneal Regenerative drugs: equipment and Protocols offers a concise evaluate of crucial recommendations within the box of corneal regenerative drugs, highlighting novel thoughts to steer the administration of key cures inside of this quarter of drugs. Divided into 4 handy sections, subject matters contain the identity, characterisation and cultivation of LESC, in addition to the research of biopolymers used because the foundation for corneal substitutes. Written within the winning Methods in Molecular Biology sequence structure, chapters contain introductions to their respective themes, lists of the required fabrics and reagents, step by step, simply reproducible protocols, and notes on troubleshooting and averting identified pitfalls.

Authoritative and simply available, Corneal Regenerative drugs: equipment and Protocols covers the basic thoughts worthy for either the laboratory and scientific settings.

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Kolli S, Ahmad S, Lako M, Figueiredo F (2010) Successful clinical implementation of corneal epithelial stem cell therapy for treatment of unilateral limbal stem cell deficiency. Stem Cells 28:597–610 183. Egbert PR, Lauber S, Maurice DM (1977) A simple conjunctival biopsy. Am J Ophthalmol 84:798–801 184. Singh R, Joseph A, Umapathy T, Tint NL, Dua HS (2005) Impression cytology of the ocular surface. Br J Ophthalmol 89:1655–1659 185. Thiel MA, Bossart W, Bernauer W (1997) Improved impression cytology techniques for the immunopathological diagnosis of superficial viral infections.

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Thoft RA (1979) Conjunctival transplantation as an alternative to keratoplasty. Ophthalmology 86:1084–1092 199. Sorsby A, Symons HM (1946) Amniotic membrane grafts in caustic burns of the eye: burns of the second degree. Br J Ophthalmol 30:337–345 200. Barraquer J (1965) The Cornea World Congress. Butterworths, Washington 201. Thoft RA (1977) Conjunctival transplantation. Arch Ophthalmol 95:1425–1427 202. Thoft RA (1984) Keratoepithelioplasty. Am J Ophthalmol 97:1–6 203. Kenyon KR, Tseng SC (1989) Limbal autograft transplantation for ocular surface disorders.

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