Complex Carbohydrates Part E by Victor Ginsburg (Eds.)

By Victor Ginsburg (Eds.)

The significantly acclaimed laboratory ordinary, Methods in Enzymology, is likely one of the such a lot hugely revered courses within the box of biochemistry. due to the fact that 1955, every one quantity has been eagerly awaited, usually consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless correct this present day - really an important booklet for researchers in all fields of existence sciences

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Res. 72, 257 (1979). 23 j. Finne, T. Krusius, and H. Rauvala, Carbohydr. Res. 80, 336 (1980). 24 L. R. Phillips and B. A. Fraser, Carbohydr. Res. 911, 149 (1981). 25 j. Paz Parente, P. Cardon, J. Montreuil, B. Fournet, and G. Ricart, Carbohydr. Res. 141, 41 (1985). [2] M I C R O S C A LMETHYLATION E ANALYSIS OF GLYCOLIPIDS 17 with use of a heating block. 5 N NaOH. Absolute EtOH is added, and the contents are dried under N2 stream at - 3 5 °, with additional EtOH as necessary to evaporate H20 by azeotropic effect.

Biochem. 31, 185 (1975). 10 j. N. BeMiller, Ado. Carbohydr. Chem. 22, 25 (1967). tt I, J. Goldstein, G. W. Hay, B. A. Lewis, and F. Smith, Methods Carbohydr. Chem. 5, 361 (1965L ~2j. E. Shively and H. E. Conrad, Biochemistry 15, 3932 (1976). ~3 R. D. Guthrie and J. F. McCarthy, Adv. Carbohydr. Chem. 22, ! 1 (1967). ~4 S. J. Angyal and K. James, Aust. J. Chem. 23, 1209 (1970). 15 B. Lindberg, J. L6nngren, and J. L. Thompson, Carbohydr. Res. 28, 351 (1973). 16 S. Svensson, this series, Vol. 50, p.

Trescony, and G. R. Gray, Carbohydr. Res. 125, 301 (1984). 30 ANALYTICALMETHODS [3] linkages. Given below are the structures of the outer-chain mannans from these y e a s t s and the products expected to be produced by sequential perethylation and reductive cleavage. RZ 3 R2" R3"R4-R6~I:t 4 RZ-Ac, R3,R4-R6,Et 5 R3-A©,Ra, R4,R4-E! R3,R4, Et 7 R2-RG-A¢,R3tR4,,Et 8 R3tRG-A¢,R2=R4-1:I When reductive cleavage was carried out with TMSOTf as the catalyst, the expected 1,5-anhydro-D-mannitol derivatives were indeed obtained from all four mannans and they were formed in the expected molar ratios (Table I).

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