Bioreaction Engineering Principles by John Villadsen, Jens Nielsen, Gunnar Lidén

By John Villadsen, Jens Nielsen, Gunnar Lidén

Integrating contemporary learn at the body structure and modelling of bioreactions and bioreactors, the authors current a complete, unified creation to the foundations and practices of the sector. The paintings beneficial properties approximately a hundred designated layout examples and difficulties, lots of that are compatible for hands-on demonstrations on a private desktop or for improved study. The textual content will function a hugely instructive advisor for college students in bioengineering and biotechnology, in addition to biochemical, chemical, and environmental engineering.

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2) The first term on the right-hand side of Eq. 2) is obviously the free diffusion term corresponding to Eq. 1). If the partition coefficient K is so sm all that KeKs a and KeKs b are small compared with I, it is seen that the last term is larger by a factor erKe than the free diffusion term. This is the effect of the facilitated diffusion. The "saturation kinetics" indicated in the text follows from Eq. 2) Ions can be taken up by the cells by passage through channels formed by certain proteins (similar to the porins of the outer membrane in Gram-negative bacteria).

NADH formed in the cytoplasm can not pass the mitochondrial membrane, and its oxidation to NAD+ is coupled to reduction of FAD to F ADH 2 in the mitochondria. Cytoplasmic NADH therefore enters the respiratory chain at the same position as F ADH 2 , and the theoretical P /0 ratio for oxidation of cytoplasmic NADH is only 2. For calculation of the overall P /0 ratio it is therefore necessary to distinguish between reactions in the cytoplasm and those in the mitochondria, and since the ratio ofNADH formed at these two locations may vary with the operating conditions it is normally impossible to specify the value of the P /0 ratio except that theoretically it should lie between 2 and 3.

Because of the rapid adjustment of the acid dissociation equilibrium and of the equilibrium between the undissociated acid in the aqueous and the lipid phase it is, however, likely that there is a film layer close to the lipid membrane in the aqueous phase. If the acid concentration is higher inside the cell than in the extracellular medium, then there is a constant rem oval of undissociated acid to the lipid phase, and this drives the acid dissociation equilibrium toward the undissociated acid form, and most of the acid may consequently be dissolved in the lipid membrane.

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