By Romano Piras
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Fructosyl transfer from sucrose. 1. Levansucrase. Levans are polysaccharides of very high molecular weight (108) made of fructofuranosyl residues linked 2,6 with branches linked 2,1. Hestrin and coworkers (120, 121, 122) found enzymes from Bacillus 37 R. D E D O N D E R subtilis and Aerobacter levanicum which synthesize levan from sucrose: n s u c r o s e ^ = ^ : l e v a n n + glucose 17) Generally, free fructose is also formed, as are oligosaccharides (123, 124). The reversibility of the reaction has been demonstrated.
T. Wilson, A Symposium on Phosphorus Metabolism, Vol. II, Williams D. McElroy and Bentley Glass, The Johns Hopkins Press, Baltimore, (1952). 18. L. F. Leloir and E. Cabib, J. Amer. Chem. , 75, 5445 (1953). 19. C. E. Cardini, L. F. Leloir and J. Chiriboga, J. Biol. , 214, 149 (1955). 20. L. F. Leloir, Arch. Biochem. , 33, 186 (1951). 21. E. Cabib, L. F. Leloir and C. E. Cardini, J. Biol. Chem. 203, 1055 (1955). 22. E. Cabib and L. F. Leloir, J. Biol. , 206, 779 (1954). 23. G. W. Kenner, A. R. Todd and R.
The glvcosidic bond joins the anomeric carbon atom to the partner through an oxygen bridge. Two configurations, a or 3, of the glycosidic linkage exist corresponding to the anomeric configuration of the parental sugar. Non Standard Abbreviations DP : degree of polymerization CL : chain length 21 R. D E D O N D E R The most classical reaction of the glycosidic bond is hydrolysis, which is catalysed by the numerous well known glycosidases. A - O - B + H - OH^^-^A - OH + BO - H 1) in which A is a glycosyl radical and B may be a glycone or an aglycone.