Applied Tissue Engineering by Minoru Ueda (Ed.)

By Minoru Ueda (Ed.)

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34: 530,1995 5. Blackburn EH, Gall JG. A tandemly repeated sequence at the termini of the extrachromosomal ribosomal RNA genes in tetrahymena. J Mol Biol. 120: 33,1978 6. Moyzis RK, Buckingham JM, Cram LS. A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes. Proc Natl Acad Sci USA. 85: 6622,1988 7. Evans SK, Bertuch AA, Lundbald V. Telomeresand telomerase: at the end, it all comestogether. Trends Cell Bio. 19: 329,1999 8. Zakian VA. Structure and function of telomeres.

12 Applied Tissue Engineering The use of gingival fibroblasts for soft-tissue augmentation Fibroblasts were obtained from the patient’s buccal gingival tissue and maintained in DMEM plus 10% autologous human serum, and incubated at 37°C with 5% CO2. Autologous patient serum was prepared from 100–150 ml of peripheral blood. The characteristics of the cultured cells were checked by immunofluorescent microscopy for known fibroblast markers. 0 × 107 cells/ml. 0 ml syringes until use. All satisfactory assessments were performed by the patient, and the following grading scale was used at 3, 6 and 12 months after the first injection (4: Completely satisfied, 3: Satisfied, 2: No remarkable change observed, 1: Not satisfied, 0: Exacerbation).

Within 5-30 seconds, the contents gained gel-like consistency because thrombin affected the polymerization of fibrin to produce an insoluble gel (Fig. 16). Application for maxillary sinus augmentation After tooth loss, alveolar augmentation of the extensively atrophied maxillary process may be required to restore the masticatory function of the patient by means of substitute teeth anchored on dental implants. In order to obtain adequate volume of bone enough to insert dental implants, elevation of the maxillary sinus floor has been carried out as a routine clinical procedure for more than 15 years [35-39].

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