By W. David Nes
Research of Sterols and different Biologically major Steroids presents the basic education for the research of chosen sterols and steroids.
The booklet consists of chapters that overview the spectroscopic and chromatographic houses of yes sterols and steroids. The textual content additionally teaches tips to isolate and signify sterols and steroid metabolites of plant, fungal, and bug foundation. Lipoprotein research and the usage of physical-analytical thoughts are likewise provided.
Biochemists, microbiologists, and clinical physiologists will locate the booklet priceless.
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Additional resources for Analysis of Sterols and Other Biologically Significant Steroids
Physiol. B 7IB, 345. Sobus, M. , and Holmlund, C E. (1976). Extraction of Lipids from Yeast. Lipids 11, 341-348. Stafford, A. , and Nes, W. D. (1983). Biosynthesis of long-chain fatty alcohols in Phytophthora cadorum. Exp. My col. 7, 374-379. Svoboda, J. , Herbert, E. , Thompson, M. , and Feldlaufer, M. F. (1986). Selective sterol transfer in the honey bee: Its significance and relationship to other Hymenoptera. Lipids 2 1 , 97-101. Isolation and Characterization of Sterols 31 Thowsen, J. , and Schroepfer, G.
Todd Lorenz, et al. nondestructive method is advantageous because resolved mixtures can be collected after separation. Reversed-phase liquid chromatography (RPLC) employs metal columns of various dimensions that contain small spheres coated with a C l g hydrocarbon (commonly octadecylsilane) or hydrocarbons of other chain lengths. Reverse phase refers to the finding that, contrary to conventional Chromatographie methods, the more-polar sterols have retention coefficients lower than nonpolar sterols and thus elute earlier (DiBussolo and Nes, 1982).
A. (1967). Identification of plant sterols using combined GLC/Mass Spectrometry. ]. Gas Chromatogr. 5, 273-282. Kuksis, A. (1964a). Gas Chromatographie fractionation of natural steryl ester mixtures. Can. ]. Biochem. 42, 407-417. Kuksis, A. (1964b). Direct gas Chromatographie fractionation of mixed neutral lipids of natural origin. Can. J. Biochem. 42, 419—430. , and Holub, B. J. (1969). Improved quantitation of plasma lipids by direct gas-liquid chromatography. /. Lipid Res. 10, 660-667. , Myher, J.