2011-2012 Basic and Clinical Science Course, Section 4: by Robert H. Rosa Jr., MD

By Robert H. Rosa Jr., MD

This quantity is split into components: half I, Ophthalmic Pathology; and half II, Intraocular Tumors: medical points. half I makes use of a hierarchy that strikes from basic to precise to assist derive a differential analysis for a particular tissue. half II is a compilation of chosen medical facets of value to the overall ophthalmologist. Following half II are the yank Joint Committee on melanoma 2010 staging kinds for ocular and adnexal tumors. This revised textual content includes a variety of new pathologic and scientific pictures. significant revision 2011-2012.

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Additional resources for 2011-2012 Basic and Clinical Science Course, Section 4: Ophthalmic Pathology and Intraocular Tumors (Basic & Clinical Science Course)

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Previous communication w ith ophthalmic pathologist to discuss a. Differential diagnosis b. Fixative (fresh vs alcohol vs glutaraldehyde vs oth er) c. Logistics of the biopsy i. Time and date (availabi lity of specia lized personnel) ii. Geographic proxim ity to laboratory chromogranin and synapto phys in fo r neuroendocrine lesions (metastatic carcinoid [see Fig 4-2], small cell carcinoma) leukocyte common antigen for lesions of hematopoi etic origin (leukemia, lymphoma) CD ant igens for subtypi ng white blood cells Her2 eu and c-Kit for prognosis and treatment (metastatic breast ca rci noma, mastocytosis) CHA PTER 4: Special Procedures.

Molecular Pathology Molecular biology techniques are used increasingly in diagnostic ophthalmiC pathology and extensively in experimental pathology (Table 4-2). More recentl y, their use has expanded to include prognostication of disease and determination of treatment. Molecular path olog y is used to identify tumor-promoting or tumor-inhibiting genes (CGH, PCR, array CGH ), such as the retinoblastoma gene; and viral DNA or RNA strands, such as those seen in herpesviruses and Epstein-Barr virus (PCR, in situ hybridization [ISH ]) .

Logistics of the biopsy i. Possible adequacy check during the biopsy (intraocular tumors) ii. Fixative to be used iii. Fresh tissue for possible mo lecula r diagnosi s b. Specific cytology form to be filled out Flow Cytometry 1. Previous communication with ophthalmic pathologist to discuss a. Fresh tissue is critical. b. Adequate samp le is essenti al. c. Geographic proximity to the laboratory Molecu lar Techniqu es and Elect ron M icroscopy 1. Previous communication w ith ophthalmic pathologist to discuss a.

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